sate slotAn x16 slot is the largest PCIe slot and ideally connects to 16 PCIe lanes. However, in many cases, you can find a PCIe x16 slot with a physical size of a full x16 slot, but in reality, it would only be connected to four PCIe lanesThe second M.2 slot usually does run through the chipset, which is also where the SATA controller for ports >5 usually does reside. And sometimes yes using the lower M.2 will disable